Journal: RMD Open

Article Title: Immunoglobulins G from Patients with Systemic Sclerosis Modify the Molecular Signatures of Endothelial Cells

doi: 10.1136/rmdopen-2024-004290

Figure Lengend Snippet: Targeted proteomics by automated quantification proteomics approach further characterised ATA+(a) and ATA+(b) group (validation cohort). ( A ) Heatmap represents selected proteins expression among groups. ( B ) Boxplots represent detailed overexpressed proteins in ATA+(a) group both vs ATA+(b) and HC ( B ). NS: non-significant (p value>0.05); *p value<0.05; **p value<0.01; ***p value<0.001; ***p value<0.0001. ATA+, anti-topoisomerase-I positive patients (N=24); ATA+(a), ATA+group a (N=11); ATA+(b), ATA+group b (N=13); ACA+, anti-centromere positive patients (N=18); ARA+, anti-RNA polymerase-III positive patients (N=5); ATA+, anti-topoisomerase-I positive patients; ATA+ (a) , anti-topoisomerase-I positive patients group a; ATA+ (b) , anti-topoisomerase-I positive patients group b; CDH2, cadherin 2; CDH5, VE-cadherin; CISD1, CDGSH iron sulphur domain 1; EIF2B3, eukaryotic translation initiation factor 2B subunit gamma; EPS15, epidermal growth factor receptor pathway substrate 15; FKBP11, FKBP prolyl isomerase 11; HC, healthy controls (N=20); ICAM-1, intercellular adhesion molecule 1; ITGA3, integrin alpha-3; NRBP1, nuclear receptor-binding protein; P4HA2, prolyl 4-hydroxylase subunit alpha-2; PNN, desmosome associated protein; RAE1, Rae1 RNA export 1; SEL1L, adaptor subunit of SYVN1 ubiquitin ligase; SNRPA, small nuclear ribonucleoprotein polypeptide A; STAM, signal transducing adaptor molecule; STK24, serine/threonine-protein kinase 24; TPP1, tripeptidyl-peptidase 1; TTN, titin.

Article Snippet: Boxplots and heatmap for AQUA peptides analysis were performed with GraphPad Prism (V.10.1.0, San Diego, California, USA).

Techniques: Targeted Proteomics, Biomarker Discovery, Expressing, Binding Assay, Ubiquitin Proteomics